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KMID : 1005219940090030221
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Journal of Embryo Transfer
1994 Volume.9 No. 3 p.221 ~ p.227
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Viability of In Vitro Fertilized Bovine Embryos Following In Vitro Culture and Embryo Transfer
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정희태
유재원/박연수/양부근/김정익
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Abstract
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This study was conducted to examine the condition of in vitro culture system and the viability after embryo transfer of in vitro matured-in vitro fertilized (IVM-IVF) bovine embryos. The in vitro development to the blastocyst stage was enhanced by supplying bovine serum albumin(BSA) to co-culture medium with bovine oviduct epithelial tissue(BOET) compared with that in medium supplemented with fetal bovine serum(FBS) (41.2% vs. 26. 3%, P<0.05). After transfer of IVM-IVF blastocysts into the uterine horn of recipient females (Aberdeen Angus), one was pregnant to term and produced a head of male Korean native calf. These results confirm that the in vitro development of IVM-IVF bovine embryos is affected with different protein source in co-culture with BOET, and IVM-IVF embryos can develop to term after in vitro culture and embryo transfer.
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KEYWORD
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